Laboratory for Preclinical Testing of Medical Devices and Cosmetic Products
Department of Medical Chemistry
Faculty of Medicine
ABOUT US
A Laboratory for Preclinical Testing of Medical Devices and Cosmetic Products has been established at the Department of Medical Chemistry of the Medical University of Gdańsk. Preclinical studies are a fundamental stage in the development process of new therapies, medications or preparations playing a key role in assessing the efficacy and safety of the studied substances, before they enter the next phases of clinical trials. The long-standing practice and experience of the unit’s scientific staff in in silico and in vitro testing allows for efficient evaluation of the cytotoxic properties and molecular mechanisms of studied substances, medical devices and cosmetic formulations, based on both standard and innovative research protocols.
The unit has laboratories and equipment to conduct research on material isolated from humans and animals, including primary cells. We have two cell laboratories, including one with higher standards, prepared for the cultivation of genetically modified organisms.
SCOPE OF TESTING
- Cytotoxicity analyses based on MTT, XTS, XTT, WST-1 protocols, among others
- Cytometric analyses, including determination of intracellularly generated reactive oxygen and nitrogen species levels, as well as type of cell death
- Western Blot analysis
- Analysis of the cellular antimigratory effect based on real-time microscopy
- Computational analyses, including molecular modeling, determination of ligand-protein interaction site and strength, molecular dynamics simulations
- Research on oxidative stress and the effects of antioxidants on cells
- Preparation of biological specimens for further testing
OUR EQUIPMENT POTENTIAL
RESEARCH TEAM
The research team includes:
- Prof. Dr. Habil. Magdalena Górska-Ponikowska
- Dr. Habil. Alicja Kuban Jankowska, Assoc. Prof.
- Narcyz Knap, Ph.D.
- Tomasz Kostrzewa, Ph.D.
- Anna Jakubczyk-Słabicka, M.A.
- Michał Suchecki, M.Eng
- Katarzyna Uzdrowska, M.Eng
PUBLICATIONS
Many publications have been developed based on the results of the research obtained by using the knowledge, experience of scientists from the Laboratory and its equipment, including.
1. Kamm A., GULCZYŃSKI JACEK, IŻYCKA-ŚWIESZEWSKA EWA, Lipinska L., GÓRSKA-PONIKOWSKA MAGDALENA. The cytotoxic properties of graphene derivatives and 2-metoxyestradiol / graphene compound on human melanoma (A375) and osteosarcoma (143B) cell lines. Virchows Arch. – 2022 : vol. 481, suppl. 1, s. S340
2. GÓRSKA-PONIKOWSKA MAGDALENA, BASTIAN PAULINA, ZAUSZKIEWICZ-PAWLAK AGATA, PŁOSKA AGATA, ZUBRZYCKI ADRIAN, KUBAN-JANKOWSKA ALICJA, Nussberger Stephan, KALINOWSKI LESZEK, KMIEĆ ZBIGNIEW. Regulation of mitochondrial dynamics in 2-methoxyestradiol-mediated osteosarcoma cell death. Sci. Rep. – 2021 : vol. 11, art. ID 1616, s. 1-12
3. KOSTRZEWA TOMASZ, Nowak Izabela, Feliczak-Guzik Agnieszka, Drzeżdżon Joanna, Jacewicz Dagmara, GÓRSKA-PONIKOWSKA MAGDALENA, KUBAN-JANKOWSKA ALICJA. Encapsulated oxovanadium(IV) and dioxovanadium(V) complexes into solid lipid nanoparticles increase cytotoxicity against MDA-MB-231 cell line. Int. J. Nanomed. 2023 : vol. 18, s. 2507-2523
4. BASTIAN PAULINA EMILIA, DACA AGNIESZKA, PŁOSKA AGATA, KUBAN-JANKOWSKA ALICJA, KALINOWSKI LESZEK, GÓRSKA-PONIKOWSKA MAGDALENA. 2-Methoxyestradiol damages DNA in glioblastoma cells by regulating nNOS and heat shock proteins. Antioxidants – 2022 : vol. 11, nr 10, art. ID 2013, s. 1-20
5. UZDROWSKA KATARZYNA, GÓRSKA-PONIKOWSKA MAGDALENA. Preservatives in cosmetics technology. Aesth. Cosmetol. Med. 2023 : vol. 12, nr 2, s. 73-78
6. Karkucinska-Wieckowska Agnieszka, Simoes Ines C. M., Kalinowski Piotr, Lebiedzinska-Arciszewska Magdalena, Zieniewicz Krzysztof, Milkiewicz Piotr, GÓRSKA-PONIKOWSKA MAGDALENA, Pinton Paolo, Malik Afshan N., Krawczyk Marcin, Oliveira Paulo J., Wieckowski Mariusz R. Mitochondria, oxidative stress and nonalcoholic fatty liver disease: A complex relationship. Eur. J. Clin. Invest. – 2022 : vol. 52, nr 3, art. ID e13622, s. 1-19
7. MUSIAŁ CLAUDIA, KNAP NARCYZ, ZAUCHA RENATA, BASTIAN PAULINA, Barone Giampaolo, Lo Bosco Giosuè, Lo-Celso Fabrizio, KONIECZNA LUCYNA, BELKA MARIUSZ, BĄCZEK TOMASZ, Gammazza Antonella Marino, KUBAN-JANKOWSKA ALICJA, Cappello Francesco, Nussberger Stephan, GÓRSKA-PONIKOWSKA MAGDALENA. Induction of 2-hydroxycatecholestrogens O-methylation: A missing puzzle piece in diagnostics and treatment of lung cancer Redox Biol. – 2022 : vol. 55, art. ID 102395, s. 1-13.
8. KOSTRZEWA TOMASZ, Wołosewicz Karol, Jamrozik Marek, Drzeżdżon Joanna, Siemińska Julia, Jacewicz Dagmara, GÓRSKA-PONIKOWSKA MAGDALENA, Kołaczkowski Marcin, Łaźny Ryszard, KUBAN-JANKOWSKA ALICJA. Curcumin and its new derivatives: correlation between cytotoxicity against breast cancer cell lines, degradation of PTP1B phosphatase and ROS generation. Int. J. Mol. Sci. – 2021 : vol. 22, nr 19
9. KOSTRZEWA TOMASZ, Jończyk Jakub, Drzeżdżon Joanna, Jacewicz Dagmara, GÓRSKA-PONIKOWSKA MAGDALENA, Kołaczkowski Marcin, KUBAN-JANKOWSKA ALICJA. Synthesis, in vitro and computational studies of PTP1B phosphatase inhibitors based on oxovanadium(IV) and dioxovanadium(V) complexes. Int. J. Mol. Sci. – 2022 : vol. 23, nr 13
10. UZDROWSKA KATARZYNA, GÓRSKA-PONIKOWSKA MAGDALENA. Sunscreen cosmetics as basic protection against photoaging. Aesth. Cosmetol. Med. 2022 : vol. 11, nr 6, s. 225-230
11. PRZYCHODZEŃ PAULINA, KUBAN-JANKOWSKA ALICJA, Wyszkowska Roksana, Barone Giampaolo, Lo Bosco Giosuè, Lo Celso Fabrizio, KAMM ANNA, DACA AGNIESZKA, KOSTRZEWA TOMASZ, GÓRSKA-PONIKOWSKA MAGDALENA. PTP1B phosphatase as a novel target of oleuropein activity in MCF-7 breast cancer model. Tixicol. Vitro. 2019 : vol. 61, art. nr 104624
PRINCE LIST
The laboratory will perform analyses according to individual orders, with customization to the specific needs of contractors on a service business or co-participation basis.
CONTACT
Prof. Magdalena Górska-Ponikowska
Department of Medical Chemistry
Medical University of Gdańsk
Dębinki 1 Street
80-211 Gdańsk
Phone: 58 349 14 50 (Secretariat of the Department)
E-mail magdalena.gorska-ponikowska@gumed.edu.pl
photo Paweł Sudara/MUG and private archive