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Forensic Analysis Laboratory

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Forensic Analysis Laboratory at the Department of Forensic Medicine

Department of Forensic Medicine

Faculty of Medicine


ABOUT US

The profile of the Department of Forensic Medicine includes thanatology, forensic medical certification, reconstruction of fatal accidents, forensic toxicology, identification of human and animal biological traces, kinship determination and personal identification, population genetics, and research on the pathomechanisms of mental disorders and processes leading to suicide. This makes it possible, e.g., to assist law enforcement and the judiciary in forensic medical decisions. More at https://structure.mug.edu.pl/85.

OUR POTENTIAL

AVAILABLE EQUIPMENT

– Liquid chromatograph coupled to a tandem mass spectrometer (LC-MS/MS 1260 Infinity – 6420 Triple Quad LC/MS)

– Gas chromatographs coupled to a mass spectrometer:

  • GC-MS 5977B MSD with electrospray ionization (EI) with GERSTEL MPS robotic system enabling injection of liquid samples and working with SBSE and SPME techniques
  • GC-MS 5975C with chemical ionization (CI)

– 2 gas chromatographs with FID detectors (possibility of injecting liquid samples and HS supersurface phase analysis)


SCOPE OF SERVICES

– securing research material during forensic medical autopsy (body fluids-5mL, small fragments tissue-1g) new planned research projects in the scope determined by a positive opinion (decision) of the Independent Commission on Bioethics and Scientific Research of the Medical University of Gdansk

– labeling and storage of protected research material (Haier Ultra Low Temperature (ULT) Freezer, deep freezer is located in the KiZMS building of MUG) of newly planned research projects within the scope defined by the positive opinion (decision) of the Independent Committee on Bioethics and Research of the Medical University of Gdańsk

– mechanical homogenization of tissue material (Qiagen TissueRuptor Handheld, homogenizer is located in the KiZMS building of MUG)

– grinding of bone and tooth material in liquid nitrogen (6850 Freezer/Mill)

– isolation of DNA from body fluids, tissue material, bones, teeth, paraffin blocks and histopathology preparations

– RNA isolation from body fluids, tissue material and paraffin blocks

– analysis of DNA fragment length polymorphism (e.g., STR markers) using PCR with starters labeled fluorescent and electrophoresis capillary (thermocyclers from Applied Biosystems, Eppendorf, Biometra, 3130 Genetic Analyzer)

– Sanger sequencing of short DNA fragments using capillary electrophoresis (3130 Genetic Analyzer)

– analysis of SNP polymorphism, CNV and gene expression levels using real-time PCR technique (7900HT Fast Real-Time PCR System)

– development and validation of analytical methods for the determination of xenobiotics (and especially psychoactive substances) in samples of biological materials and analysis of non-biological materials (dried matter, powders, tablets)

– potential newly planned scientific research projects in cooperation with MUG units (detailed information via email contact of core facility coordinator)


DOMESTIC AND FOREIGN COOPERATION (SELECTED EXAMPLES)

  • Institute of Forensic Medicine in Muenster, Germany
  • Prof. Dr. Jan Sehn Institute of Forensic Expertise in Krakow
  • Fakultät für Naturwissenschaften Institut für Biologie (IBIO) Otto-von-Guericke-Universität Magdeburg
  • Pomeranian Center of Toxicology (PCT) in Gdansk and the Department of Clinical Toxicology (ZTK) MUG
  • Forensic Laboratory of the Voivodeship Police Headquarters (LK KWP) in Gdansk
  • Archiwum X (entire Poland)
  • Scientific and Practical Center of the State Committee of Forensic Expertise in Minsk (Belarus)
  • University Medical Center of Erasmus University in Rotterdam, the Netherlands


SELECTED PUBLICATIONS

Publications that have been developed by using the research methods described:

1. Ribosomal DNA transcription is increased in the left nucleus accumbens of heroin‑dependent males – Tomasz Gos, Johann Steiner,Kurt Trübner, Marta Krzyżanowska, Michał Kaliszan. Vol.:(0123456789)13 European Archives of Psychiatry and Clinical Neuroscience (2022) 272:1603–1609, https://doi.org/10.1007/s00406-022-01423-7

2. The left‑lateralisation of citrate synthase activity in the anterior cingulate cortex of male violent suicide victims. Karol Karnecki, Julian Świerczyński, Johann Steiner, Marta Krzyżanowska, Michał Kaliszan, Tomasz Gos. European Archives of Psychiatry and Clinical Neuroscience https://doi.org/10.1007/s00406-022-01509-2

3. Long-Term Stability of Benzodiazepines and Z-Hypnotic Drugs in Blood Samples Stored at Varying Temperatures – Laura Banaszkiewicz, MATEUSZ KACPER WOŹNIAK, EWA DOMAGALSKA, MICHAŁ KALISZAN, Agata Kot-Wasik. – Journal of Analytical Toxicology, dostęp online 01.02.2022, bkac006, https://doi.org/10.1093/jat/bkac006

4. Potential of DNA zygosity tests for non-invasive evaluation of risk of complications in twin pregnancies – DZIENNIK AGNIESZKA, PREIS KRZYSZTOF, Swiatkowska-Freund Malgorzata, RĘBAŁA KRZYSZTOF. – Ginekol. Pol. – 2022 : vol. 93, nr 4, s. 310-313, bibliogr. 29 poz., streszcz. ang. Selected political criminal poisonings in the years 1978-2020: detection and treatment – Brunka Zuzanna, Ryl Jan, Brushtulli Piotr, Gromala Daria, Walczak Grzegorz, ZIĘBA SONIA, PIEŚNIAK DOROTA, SEIN ANAND JACEK, WIERGOWSKI MAREK. – Toxics – 2022 : vol. 10, nr 8, art. ID 468, s. 1-23, bibliogr. 83 poz., streszcz. ang.

5. Clinical anatomy of the spatial structure of the right ventricular outflow tract – KACZYŃSKA AGATA EWA, KOSIŃSKI ADAM, Bobkowska Katarzyna, ZAJĄCZKOWSKI MIŁOSZ ANDRZEJ, KAMIŃSKI RAFAŁ, PIWKO GRZEGORZ MAREK, GLEINERT-ROŻEK MARTA, GOS TOMASZ, KARNECKI KAROL, KOZŁOWSKI DARIUSZ. – Adv. Clin. Exp. Med. – 2022 : vol. 31, nr 1, s. 33-40, bibliogr. 19 poz., streszcz. ang.

6. Arterial hypertension and remodelling of the right ventricle – KOSIŃSKI ADAM, PIWKO GRZEGORZ M., KAMIŃSKI RAFAŁ, Nowicka E., KACZYŃSKA AGATA, ZAJĄCZKOWSKI MIŁOSZ, CZERWIEC KATARZYNA, GLEINERT-ROŻEK MARTA, KARNECKI KAROL, GOS TOMASZ. – Folia Morphol. – 2022 : vol. 81, nr 2, s. 336-342, bibliogr. 27 poz., streszcz. ang.

7. miRNA profiles change during grass pollen immunotherapy irrespective of clinical outcome – SPECJALSKI KRZYSZTOF, MACIEJEWSKA AGNIESZKA, ROMANTOWSKI JAN, PAWŁOWSKI RYSZARD, JASSEM EWA, NIEDOSZYTKO MAREK. – Immunotherapy – 2022 : vol. 14, nr 6, s. 433-444, bibliogr. 65 poz., streszcz. ang.

8. Reduced GABAergic neuropil and interneuron profiles in schizophrenia: complementary analysis of disease course-related differences – Förster Antonia, Model Vera, GOS TOMASZ, Frodl Thomas, Schiltz Kolja, Dobrowolny Henrik, Meyer-Lotz Gabriela, Guest Paul C., Mawrin Christian, Bernstein Hans-Gert, Bogerts Bernhard, Schlaaff Konstantin, Steiner Johann. – J. Psychiatr. Res. – 2022 : vol. 145, s. 50-59, bibliogr. poz., streszcz. ang.

9. Comparative study of the percutaneous permeation and bioaccumulation of a cyclic siloxane using frozen-thawed and nonfrozen ex vivo human skin – MOJSIEWICZ-PIEŃKOWSKA KRYSTYNA, Krenczkowska Dominika, BAZAR DAGMARA, WIELGOMAS BARTOSZ, CAL KRZYSZTOF, KALISZAN MICHAŁ. – Toxicol. Vitro – 2022 : vol. 82, art. ID 105379, s. 1-9, bibliogr. poz., streszcz. ang.

10. Fatal suicidal intoxication with pentoxifylline complicated by cardiovascular disorders – SEIN ANAND JACEK, WIERGOWSKI MAREK, WIŚNIEWSKI MAREK ROMAN, KOSMOWSKA MONIKA, KATA MARZENA, WOŹNIAK MATEUSZ KACPER. – Toxics – 2022 : vol. 10, nr 8, art. ID 447, s. 1-9, bibliogr. 7 poz., streszcz. ang. Time of death estimation based on the analysis of tanathochemical processes in forensic medicine – ZIĘBA SONIA, WIERGOWSKI MAREK, KRZYŻANOWSKA MARTA, GOS TOMASZ, Sołtyszewski Ireneusz. – Rom. J. Leg. Med. – 2021 : vol. 29, nr 3, s. 261-271, bibliogr. 58 poz., streszcz. ang.

11. Development and validation of a method for the simultaneous analysis of fatty acid ethyl esters, ethyl sulfate and ethyl glucuronide in neonatal meconium: application in two cases of alcohol consumption during pregnancy – WOŹNIAK MATEUSZ KACPER, Banaszkiewicz Laura, Aszyk Justyna, WIERGOWSKI MAREK, JAŃCZEWSKA IWONA, WIERZBA JOLANTA, Kot-Wasik Agata, Biziuk Marek. – Anal. Bioanal. Chem. – 2021 : vol. 413, nr 11, s. 3093-3105, bibliogr. 18 poz., streszcz. ang.

12. AgNOR parameters of dorsal raphe nucleus neurons as a potential diagnostic tool which could aid the differentiation between suicidal and non-suicidal death – KRZYŻANOWSKA MARTA, Steiner Johann, GOS TOMASZ. – Eur. Arch. Psychiatr. Clin. Neurosci. – 2021 : vol. 271, nr 3, s. 587-589, bibliogr. 6 poz.

13. Reduced habenular volumes and neuron numbers in male heroin addicts: a post-mortem study – Müller Ulf J., Ahrens Morltz, Vasilevska Veronica, Dobrowolny Henrik, Schiltz Kolja, Schlaaff Konstantin, Mawrin Christian, Frodl Thomas, Bogerts Bernhard, GOS TOMASZ, Truebner Kurt, Bernstein Hans-Gert, Steiner Johann. – Eur. Arch. Psychiatr. Clin. Neurosci. – 2021 : vol. 271, nr 5, s. 835-845, bibliogr. 51 poz., streszcz. ang.

14. The role of microglia in neuropsychiatric disorders and suicide – Brisch Ralf, WOJTYLAK SZYMON, Saniotis Arthur, Steiner Johann, GOS TOMASZ, Kumaratilake Jaliya, Henneberg Maciej, Wolf Rainer. – Eur. Arch. Psychiatr. Clin. Neurosci. – 2021 : vol. 272, nr 6, s. 929-945, bibliogr. 236 poz., streszcz. ang.

15. Reduced ribosomal DNA transcription in the prefrontal cortex of suicide victims : consistence of new molecular RT-qPCR findings with previous morphometric data from AgNOR-stained pyramidal neurons – KRZYŻANOWSKA MARTA, RĘBAŁA KRZYSZTOF, Steiner Johann, KALISZAN MICHAŁ, PIEŚNIAK DOROTA, KARNECKI KAROL, WIERGOWSKI MAREK, Brisch Ralf, Braun Katharina, JANKOWSKI ZBIGNIEW, KOSMOWSKA MONIKA, Chociej Joanna, GOS TOMASZ. – Eur. Arch. Psychiatr. Clin. Neurosci. – 2021 : vol. 271, nr 3, s. 567-576, bibliogr. 67 poz., streszcz. ang.

16. Epidemiology of suicide in the Tricity metropolitan area in northern Poland 1980-2009: evidence of influence by political and socioeconomic changes – KARNECKI KAROL, Steiner Johann, Guest Paul C., KRZYŻANOWSKA MARTA, Mańkowski Dobrosław, GOS TOMASZ, KALISZAN MICHAŁ. – Forensic Sci. Int. Rep. – 2021 : vol. 3, art. ID 100219, s. 1-7, bibliogr. 32 poz., streszcz. ang.

17. Fake gunshot wounds in the skull: post-mortem artifact caused by steel probe during police search for a missing body – KALISZAN MICHAŁ, DALEWSKI WOJCIECH, DAWIDOWSKA JOANNA, GOS TOMASZ, JANKOWSKI ZBIGNIEW. – Int. J. Legal Med. – 2021 : vol. 135, nr 3, s. 879-883, bibliogr. 19 poz., streszcz. ang.

18. Fatal N-ethylhexedrone intoxication – DOMAGALSKA EWA, Banaszkiewicz Laura, WOŹNIAK MATEUSZ KACPER, KATA MARZENA, SZPIECH BEATA, KALISZAN MICHAŁ. – J. Anal. Toxicol. – 2021 : vol. 45, nr 6, s. e1-e6, bibliogr. 13 poz.

19. The application of metabolomics in forensic science with focus on forensic toxicology and time-of-death estimation – DAWIDOWSKA JOANNA, KRZYŻANOWSKA MARTA, MARKUSZEWSKI MICHAŁ JAN, KALISZAN MICHAŁ. – Metabolites – 2021 : vol. 11, nr 12, art. ID 801, s. 1-20, bibliogr. 115 poz., streszcz. ang.

20. Significance of interleukin-31 (IL-31) gene polymorphisms and IL-31 serum level in psoriasis in correlation with pruritus – PURZYCKA-BOHDAN DOROTA, Gleń Jolanta, ZABŁOTNA MONIKA, NEDOSZYTKO BOGUSŁAW, SZCZERKOWSKA-DOBOSZ ANETA, SOKOŁOWSKA-WOJDYŁO MAŁGORZATA, RĘBAŁA KRZYSZTOF, NOWICKI ROMAN J.. – Post. Dermatol. Alergol. – 2021 : t. 38, nr 4, s. 657-664, bibliogr. 32 poz., streszcz. ang.

21. IFNG, FCER1A, PCDHB10 expression as a new potential marker of efficacy in grass pollen allergen-specific immunotherapy – ROMANTOWSKI JAN, MACIEJEWSKA AGNIESZKA, Polanska Joanna, Wasilewska Eliza, SPECJALSKI KRZYSZTOF, CHEŁMIŃSKA MARTA, JASSEM EWA, NIEDOSZYTKO MAREK. – Post. Dermatol. Alergol. – 2021 : t. 38, nr 4, s. 665-672, bibliogr. 55 poz., streszcz. ang.


PRICE LIST

The laboratory performs research and analysis according to individual projects. Service costs are valued individually.


CONTACT

Marta Krzyżanowska, Ph.D.
Department of Forensic Medicine
Medical University of Gdańsk
Dębowa 23
80-211 Gdańsk

Phone 58 349 12 50 (Secretariat)
E-mail marta.krzyzanowska@gumed.edu.pl