Physical Activity Metabolism Laboratory

Division of Bioenergetics and Physiology of Exercise

Faculty of Health Sciences with the Institute of Maritime and Tropical Medicine

ABOUT US

The Division of Bioenergetics and Physiology of Exercise, which houses the Physical Activity Metabolism Laboratory. It is a state-of-the-art research unit focusing on studies aimed at preserving, enhancing and maintaining broadly defined vis vitalis – the force of life. Head of the Division, Prof. Jędrzej Antosiewicz has many years of experience gained from research grants and cooperation with centers at home and abroad.

The unit has equipment that allows research onhuman and animal material, as well as experiments strictly under cell culture conditions. The department has the capacity to study the broadly defined physiology and biochemistry of physical activity in humans and conduct research on animal models.

Analytical techniques used in the Laboratory employ flow cytometry technology, Magpix Luminex xmap device, Western Blot assay technique, Elisa immunoenzymatic assays, cell viability assays and cytotoxic activity of compounds (SRB Test), as well as preparation of biological specimens for further examinations involving steps such as separation of material specific for planned assays, including preservation of stable genetic material or using cryogenic conditions. The laboratory also carries out projects involving multi-week experiments on a large population with accompanying detailed analyses.

AVAILABLE EQUIPMENT AND DEVICES

• Magpix Luminex Xmap – an instrument using flow cytometry for simultaneous analysis of up to 100 determinations from a single sample
• Optima XPN-80 Ultracentrifuge at maximum speed of 80.000 rpm, 548300 xg
• Prod. Bio Rad equipment set for Western Blot analysis together with fluorescence reading on ImageQuant LAS 500
• Laboratory equipped forcell culture: laminar Bioair, incubator, bath, Luna II cell count analyze=
• Nautilus and Monarch measurement bikes, digital dynamometer, ammonia and lactic acid level analyzer
• Equipment for field studies, such as the Zenithlab portable centrifuge, Dometic portable refrigerator

PUBLICATIONS

Publications based on research results obtained using our research equipment:

1. Borkowska, A., Popowska, U., Spodnik, J., Herman-Antosiewicz, A., Wozniak, M., and Antosiewicz, J. (2020a). JNK/p66Shc/ITCH Signaling Pathway Mediates Angiotensin II-induced Ferritin Degradation and Labile Iron Pool Increase. Nutrients 12. doi:10.3390/nu12030668

2. Borkowska, A., Tomczyk, M., Zychowska, M., Pilis, W., Zych, M., and Antosiewicz, J. (2021a). Effect of 8-Day Fasting on Leukocytes Expression of Genes and Proteins Involved in Iron Metabolism in Healthy Men. Int J Mol Sci 22. doi:10.3390/ijms22063248

3. Borkowska, A., Ziolkowski, W., Kaczor, K., Herman-Antosiewicz, A., Knap, N., Wronska, A., and Antosiewicz, J. (2020b). Homocysteine-induced decrease in HUVEC cells’ resistance to oxidative stress is mediated by Akt-dependent changes in iron metabolism. European Journal of Nutrition. doi:10.1007/s00394-020-02360-8

4. Kaczor-Keller, K.B., Pawlik, A., Scianowski, J., Pacula, A., Obieziurska, M., Marcheggiani, F., Cirilli, I., Tiano, L., and Antosiewicz, J. (2020). In Vitro Anti-Prostate Cancer Activity of Two Ebselen Analogues. Pharmaceuticals (Basel) 13. doi:10.3390/ph13030047

5. Kortas, J., Ziemann, E., Juszczak, D., Micielska, K., Kozlowska, M., Prusik, K., Prusik, K., and Antosiewicz, J. (2020). Iron Status in Elderly Women Impacts Myostatin, Adiponectin and Osteocalcin Levels Induced by Nordic Walking Training. Nutrients 12. doi:10.3390/nu12041129

6. Mieszkowski, J., Borkowska, A., Stankiewicz, B., Kochanowicz, A., Niespodzinski, B., Surmiak, M., Waldzinski, T., Rola, R., Petr, M., and Antosiewicz, J. (2021). Single High-Dose Vitamin D Supplementation as an Approach for Reducing Ultramarathon-Induced Inflammation: A Double-Blind Randomized Controlled Trial. Nutrients 13. doi:10.3390/nu13041280

7. Mieszkowski, J., Stankiewicz, B., Kochanowicz, A., Niespodzinski, B., Borkowska, A., and Antosiewicz, J. (2020a). Effect of Ischemic Preconditioning on Marathon-Induced Changes in Serum Exerkine Levels and Inflammation. Frontiers in Physiology 11. doi:ARTN 57122010.3389/fphys.2020.571220

8. Mieszkowski, J., Stankiewicz, B., Kochanowicz, A., Niespodzinski, B., Kowalik, T., Zmijewski, M.A., Kowalski, K., Rola, R., Bienkowski, T., and Antosiewicz, J. (2020b). Ultra-Marathon-Induced Increase in Serum Levels of Vitamin D Metabolites: A Double-Blind Randomized Controlled Trial. Nutrients 12. doi: ARTN 3629 10.3390/nu12123629

9. Tomczyk, M., Kortas, J., Flis, D., Kaczorowska-Hac, B., Grzybkowska, A., Borkowska, A., Lewicka, E., Dabrowska-Kugacka, A., and Antosiewicz, J. (2020). Marathon Run-induced Changes in the Erythropoietin-Erythroferrone-Hepcidin Axis are Iron Dependent. Int J Environ Res Public Health 17. doi:10.3390/ijerph17082781

10. Zychowska, M., Grzybkowska, A., Wiech, M., Urbanski, R., Pilch, W., Piotrowska, A., Czerwinska-Ledwig, O., and Antosiewicz, J. (2020). Exercise Training and Vitamin C Supplementation Affects Ferritin mRNA in Leukocytes without Affecting Prooxidative/Antioxidative Balance in Elderly Women. International Journal of Molecular Sciences 21. doi:ARTN 6469 10.3390/ijms21186469

PRICE LIST

The laboratory performs research and analysis according to individual projects. Service costs are valued individually.

CONTACT

Prof. Jędrzej Antosiewicz
jedrzej.antosiewicz@gumed.edu.pl

Andżelika Borkowska, Ph.D.
andzelika.borkowska@gumed.edu.pl

Ulana Juhas, Ph.D.
ulana.juhas@gumed.edu.pl

Physical Activity Metabolism Laboratory
at theDivision of Bioenergetics and Physiology of Exercise
Medical University of Gdańsk
Dębinki 1 Street
80-211 Gdańsk

Phone: 58 349 14 56