Subject Area and Research Team: X. Molecular and biochemical methods for study mechanisms of civilization diseases
Research topics
1. The molecular mechanisms determining cell fate during stress responses and their connection to human pathologies including the role of microRNA in cellular signalling networks
2. The development of RNAi therapies
3. Biomarkers in patients with diabetes and chronic vascular complications
4. Short-Chain Fatty Acids (SCFAs) as potential risk factors for metabolic syndrome and coronary artery disease
5. The role of nucleotide and adenosine metabolism in the pathophysiology of cancer and cardiovascular diseases; adenosine deaminase as a marker of endothelial dysfunction
6. Nucleoside transport inhibitors and ADA inhibitors in endothelial dysfunction therapy
Coordinators
| Coordinator #1 | Coordinator #2 | |
|---|---|---|
| Name and surname |  |
 |
| Academic degree | Prof. Dr. Habil. | Dr. Habil. |
| Employment unit | Department of Immunobiology and Environmental Microbiology | Department of Biochemistry |
| Polish Platform of Medical Research | Prof. Dr. Habil. Katarzyna Zorena | Dr. Habil. Barbara Kutryb-Zając |
| katarzyna.zorena@gumed.edu.pl | barbara.kutryb-zajac@gumed.edu.pl | |
| Phone number | +48 58 349 17 66 | +48 58 349 14 60 |
Research Team X. Molecular and biochemical methods for study mechanisms of civilization diseases
Team Members X. Molecular and biochemical methods for study mechanisms of civilization diseases (176 KB)Feel free to contact one of our coordinators to join our Research Team.
Key current projects
1. The role and mechanism of signal passing from HIFs 1 to 2 and 3 in human endothelium
2. The molecular mechanisms determining cell fate during UPR
3. The detection microbial metabolites short chain fatty acids in fecal and serum samples
4. The role of adenosine in calcific aortic valve disease and atherosclerosis
Key grants
| Funding agency/grant number | Title of the project | Years | |
|---|---|---|---|
| 1. | NCN (OPUS 19)/2020/37/B/NZ3/00861 | Deciding the cell fate during UPR – are we barking up the wrong tree? | 2021-2025 |
| 2. | NCN (SONATA BIS 5)/2015/18/E/NZ3/00687 | Molecular mechanisms of HIF switch in human endothelium | 2016-2022 |
| 3. | NCBiR | New anticancer compounds interfering function of telomeres | 2015-2022 |
| 4. | NCN/2018/31/N/NZ9/0174 | Analysis of transcriptomic and physiological changes in leaves of Brassica napus L. after the exposure to heavy metals, with particular emphasis on the application of sewage sludge on degraded soils | 2015-2022 |
| 5. | The Ministry for Europe and Foreign Affairs (Ministère de l’Europe et des Affaires étrangères), France | Optimization of metal phytoextraction using advanced toxicological methods | 2018-2020 |
| 6. | NCN (SONATA 15)/2019/35/D/NZ3/03512 | Ecto-enzymes in interactions of vascular endothelium with blood circulating cells in physiology, pathology and therapy; could cells exchange their ecto-enzymes? | 2020-2023 |
| 7. | NAWA (Polonium) BPN/BFR/2021/1/00027/U/00001 | Sewage sludge as agricultural fertilizer – green opportunity or hidden threat? Study on the potential spread of antibiotic resistance through long-term land supplementation with sewage sludge | 2022-2022 |
International cooperation
| Foreign partner (unit name) | Principal investigator(s) | Area of cooperation | |
|---|---|---|---|
| 1. | Departments: of Cell, Developmental and Integrative Biology and of Genetic, University of Alabama at Birmingham, Birmingham, USA | James Collawn David K. Crossman |
Cell biology NGS- focused bioinformatics |
| 2. | University of Lille, Sciences and Technologies Department of Biology, Laboratory of Environment, Ecology and Ecotoxicology, Villenueve d’Ascq, France |
Franck Vandenbulcke | Ecotoxicology Molecular biology including NGS and bioinformatics Field work/greenhouse experiments |
| 3. | Université de Pau et des Pays de L’Adour, E2S UPPA, CNRS, UMR IPREM 5254, Environmental Microbiology, Pau, France | Rémy Guyoneaud | Environmental microbiology – NGS, bioinformatics |
| 4. | Department of Internal Medicine II, Division of Cardiology, Medical University of Vienna, Vienna, Austria | Jolanta Siller-Matula | Thrombosis |
| 5. | University of Pavia Medical School, Pavia, Italy | Stefano De Servi | Inflammation and thrombosis |
| 6. | Department of Biology, University of Pisa, Italy | Roberto Giovannoni | Cell biology, immunofluorescence, tracking proteins |
National cooperation
| Foreign partner (unit name) | Principal investigator(s) | Area of cooperation | |
|---|---|---|---|
| 7. | Metabolomics Laboratory Clinical Research Centre, Medical University of Bialystok, Poland | Michal Ciborowski | Metabolomics |
Key publications
1. Bartoszewska S, Cabaj A, Dabrowski M, Collawn JF, Bartoszewski R. miR-34c-5p modulates X-box-binding protein 1 (XBP1) expression during the adaptive phase of the unfolded protein response. Faseb J. 2019; 33: 11541-11554.
2. Bartoszewska S, Kochan K, Piotrowski A, Kamysz W, Ochocka RJ, Collawn JF, Bartoszewski R. The hypoxia-inducible miR-429 regulates hypoxia-inducible factor-1 alpha expression in human endothelial cells through a negative feedback loop. Faseb J. 2015: 29: 1467-1479.
3. Bartoszewski R, Moszynska, A, Serocki M, Cabaj A, Polten A, Ochocka R, Dell’Italia L, Bartoszewska S, Kroliczewski J, Dabrowski MD, Collawn JF. Primary endothelial cell-specific regulation of hypoxia-inducible factor (HIF)-1 and HIF-2 and their target gene expression profiles during hypoxia. Faseb J. 2019; 33: 7929-7941.
4. Zorena K, Jachimowicz-Duda O, Wąż P. The cut-off value for interleukin 34 as an additional potential inflammatory biomarker for the prediction of the risk of diabetic complications. Biomarkers. 2016; 21(3): 276-82.
5. Koziński M, Ostrowska M, Adamski P, Sikora J, Sikora A, Karczmarska-Wódzka A, Marszałł MP, Boinska J, Laskowska E, Obońska E, Fabiszak T, Kubica J. Which platelet function test best reflects the in vivo plasma concentrations of ticagrelor and its active metabolite? The HARMONIC study. Thromb Haemost. 2016; 116(6): 1140-1149.
6. Iannopollo G, Ferlini M, Koziński M, Ormezzano MF, Crimi G, Lanfranchi L, Camporotondo R, Visconti LO, De Ferrari GM, De Servi S. Patient Outcomes With STEMI Caused by Aneurysmal Coronary Artery Disease and Treated With Primary PCI. J Am CollCardiol. 2017; 69(24): 3006-3007.
7. Jaskulak M, Grobelak A, Vandenbulcke F. Effects of sewage sludge supplementation on heavy metal accumulation and the expression of ABC transporters in Sinapis alba L. during assisted phytoremediation of contaminated sites. Ecotoxicology and Environmental Safety. 2020; 197: 110606.
8. Kutryb-Zajac B, Harasim G, jedrzejewska A, Krol O, Braczko A, Jablonska P, Mierzejewska P, Zielinski J, Slominska EM, Smolenski RT. Macrophage-derived adenosine deaminase 2 correlates with M2 macrophage phenotype in triple negative breast cancer. Int J Mol Sci. 2021; 22(7): 3764.
9. Kutryb-Zajac B, Jablonska P, Serocki M, Bulinska A, Mierzejewska P, Friebe D, Alter C, Jasztal A, Lango R, Rogowski J, Bartoszewski R, Slominska E, Chlopicki S, Schrader J, Yacoub M, Smolenski RT. Nucleotide ecto-enzyme metabolic pattern and spatial distribution in calcific aortic valve disease: its relation to pathological changes and clinical presentation. Clin Res cardiol. 2020; 109(2): 137-160.
10. Olkowicz M, Debski J, Jablonska P, Dadlez M, Smolenski RT. Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis. J Chromatogr A. 2017; 1517: 66-78.