Subject Area and Research Team: IX. Technological developments for bioan...

Subject Area and Research Team: IX. Technological developments for bioanalysis and therapy including cell based, 3D and artificial engineered models

Research topics

1. Experimental nanomedicine

2. Targeted therapy for pancreatic and breast cancer. Mechanism of resistance and progression

3. Biomaterials and regenerative medicine. Wound healing and cartilage regeneration

4. Antibacterial Peptides (AMPs) & Biosensors

5. The study of brain energy metabolism

6. Use spore as universal carriers, social interaction between B. subtilis and D. solani and bacteriophages of Clostridium dificile

7. Pharmaceutical Technology & Biopharmacy


Coordinator #1 Coordinator #2 Coordinator #3
Name and surname
Michał Pikuła
Iwona Inkielewicz-Stępniak
Rafał Sądej
Academic degree Prof. Dr. Habil. Prof. Dr. Habil. Assoc. Prof. Dr. Habil.
Employment unit Department of Embryology Department of Pharmaceutical Patophysiology Department of Molecular Enzymology and Oncology
Polish Platform of Medical Research Prof. Dr. Habil. Michał Pikuła Prof. Dr. Habil. Iwona Inkielewicz-Stępniak Assoc. Prof. Rafał Sądej, Dr. Habil.
Phone number +48 58 523 13 68 +48 58 349 15 16 +48 58 349 14 70

Research Team IX. Technological developments for bioanalysis and therapy including cell based, 3D and artificial engineered models

Team Members IX. Technological developments for bioanalysis and therapy including cell based, 3D and artificial engineered models (158 KB)

Feel free to contact our coordinator to join the Research Team.

Key current projects

1. Nanodrugs as a new approach in therapy of cardiovascular diseases, cancer and tumour-associated angiogenesis

2. Establishment of pancreatic cancer organoids model for drug and nanodrug screening

3. Analysis of FGFR-mediated regulation of autophagy in breast cancer progression and response to endocrine therapies

4. New generation bioactive molecules delivery systems, based on chemically synthesised and obtained through genetic engineering nanobiomaterials

5. Analysis of mechanisms of resistance to innovative FGFR kinases inhibitor – development of novel anti-cancer therapy

6. Antimicrobial peptides design and synthesis

7. Metabolism of adenine nucleotides in regulation of cancer progression and promotion

8. Social interaction between B. subtilis and D. solani, bacteriophages of Clostridium dificile

9. Lipid microparticles nanoparticles and lyophilized formulations

10. Ocular and transdermal penetration

Key grants

Funding agency/grant number Principal investigator(s) Title of the project Years
1. NCN, OPUS 18,
Prof. Iwona Inkielewicz-Stępniak Synthetic analogues of opioid growth factor linked to derivatives of glucosamine as a compounds with the potential for targeted therapy against pancreatic tumour 2020-2024
Prof. Iwona Inkielewicz-Stępniak Antithrombotic and antimicrobial activity of functionalized silver nanoparticles performed under in vitro, ex vivo condition and on animal model 2018-2022
3. H2020, EU-funded, Marie Skłodowska-Curie – 861196 Prof. Iwona Inkielewicz-Stępniak PRECODE Network PancREatic Cancer OrganoiDs rEsearch 2019-2023
4. NCN, OPUS 17,
Prof. M. Pikuła Effect of adipose-derived stem cells on in vitro cultured skin cells obtained from patients undergoing chemotherapy 2020-2023
5. TECHMATSTRATEG2/410747/11/NCBR2019 Prof. M. Pikuła New generation bioactive molecules delivery systems, based on chemically synthesised and obtained through genetic engineering nanobiomaterials, Bionanova 2019-2022
6. NCN, Opus 14,
Prof. R. Sądej Significance of FGFR2 in regulation of autophagy in breast carcinoma – implications for disease prognostication 2018-2021
7. NCN, OPUS 12,
Prof. Wojciech Kamysz Research on the use of new analogs of endogenous peptides in the treatment of skin infections of staphylococcal etiology 2017-2021
8. NCN, OPUS 15,
Prof. Michał Obuchowski Social interaction between B. subtilis and D. solani 2018-2021
9. NCN, OPUS 15,
Prof. Małgorzata Sznitowska Comparative analysis of structural and diffusive properties of adhesive polymer based carriers used in medicated patches applied to the skin 2019-2022

International cooperation

Foreign partner (unit name) Principal investigator(s) Area of cooperation
1. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil Ana C. A. Melo Preparation of molecular receptors (odorant binding proteins, olfactory receptors)
2. Trinity College, Dublin, Ireland Maria Jose Santos-Martinez In Vitro Microvessel Models
3. University of Birmingham, UK Fedor Berditchevski Constructs exchange, project realisation
4. Transplantation Research and Immunology Group, Nuffield Department of Surgical Sciences, University of Oxford, Oxford, England Joanna Hester Immunological studies, NAWA program scholarship
5. University of Erlangen, Nuremberg, Germany Christian Pilarsky Pancreatic Cancer Organoids
6. Department of Chemistry and Chemical Engineering,Chalmers University of Technology, Goeteborg, Sweden Anette Larsson Structural analysis of polymeric materials
7. University of Greifswald, Department of Biopharmaceutics and Pharmaceutical Technology, Center of Drug Absorption and Transport (C_DAT), Greifswald, Germany Werner Weitschies Biorelevant dissolution tests
8. University Frederico II, Naples, Italy Ezio Ricca B. subtilis spore
9. Department of Electronic Engineering; Department of Chemical Science and Technology, Tor Vergata, University of Rome, Rome, Italy Corrado Di Natale Design and construction of (bio)sensor arrays

Key publications

1. Hajtuch J, Hante N, Tomczyk E, Wojcik M, Radomski MW, Santos-Martinez MJ, Inkielewicz-Stępniak I. Effects of functionalized silver nanoparticles on aggregation of human blood platelets. Int. J. Nanomed. 2019; 14: 7399-7417.

2. Budka J, Kowalski Sz, Dzierzbicka K, Inkielewicz-Stepniak I. Opioid Growth Factor and its Derivatives as Potential Non-toxic Multifunctional Anticancer and Analgesic Compounds. Current Medicinal Chemistry. 2021; 28(4): 673-686.

3. Deptuła M, Karpowicz P, Wardowska A, Sass P, Sosnowski P, Mieczkowska A, Filipowicz N, Dzierżyńska M, Sawicka J, Nowicka E, Langa P, Schumacher A, Cichorek M, Zieliński J, Kondej K, Kasprzykowski F, Czupryn A, Janus Ł, Mucha P, Skowron P, Piotrowski A, Sachadyn P, Rodziewicz-Motowidło S, Pikuła M. Development of a Peptide Derived from Platelet-Derived Growth Factor (PDGF-BB) into a Potential Drug Candidate for the Treatment of Wounds. Adv Wound Care. 2020; 9(12): 657-675.

4. Sass P, Sosnowski P, Podolak-Popinigis J, Górnikiewicz B, Kamińska J, Deptuła M, Nowicka E, Wardowska A, Ruczyński J, Rekowski P, Rogujski P, Filipowicz N, Mieczkowska A, Peszyńska-Sularz G, Janus Ł, Skowron P, Czupryn A, Mucha P, Piotrowski A, Rodziewicz-Motowidło S, Pikuła M, Sachadyn P. Epigenetic inhibitor zebularine activates ear pinna wound closure in the mouse. EBioMedicine. 2019; 46: 317-329.

5. Piasecka D, Braun M, Mieszkowska M, Kowalczyk L, Kopczynski J, Kordek R, Sadej R, Romanska HM. Upregulation of HIF1-α via an NF-κB/COX2 pathway confers proliferative dominance of HER2-negative ductal carcinoma in situ cells in response to inflammatory stimuli. Neoplasia. 2020; 22: 576-589.

6. Braun M, Piasecka D, Tomasik B, Mieczkowski K, Stawiski K, Zielinska A, Kopczynski J, Nejc D, Kordek R, Sadej R, Romanska HM. Hormonal receptor status determines prognostic significance of FGFR2 in invasive breast carcinoma. Cancers. 2020; 12(9): 2713.

7. Kamysz E, Sikorska E, Jaśkiewicz M, Bauer M, Neubauer D, Bartoszewska S, Barańska-Rybak W, Kamysz W. Lipidated analogs of the LL-37-derived peptide fragment KR12 – structural analysis, surface-active properties and antimicrobial activity. Int. J. Mol. Sci. 2020; 2: 1-22.

8. Ronowska A, Gul-Hinc S, Michno A, Bizon-Zygmańska D, Zyśk M, Bielarczyk H, Szutowicz A, Gapys B, Jankowska-Kulawy A. Aggravated effects of coexisting marginal thiamine deficits and zinc excess on SN56 neuronal cells. Nutritional Neuroscience. 2019; 24(8): 1-11.

9. Grela A, Jamrożek I, Hubisz M, Iwanicki A, Hinc K, Kaźmierkiewicz R, Obuchowski M. Positions 299 and 302 of the GerAA subunit are important for function of the GerA spore germination receptor in Bacillus subtilis. Plos ONE. 2018; 13(6): e0198561.

10. Płaczek M, Wątróbska-Świetlikowska D, Stefanowicz-Hajduk J, Drechsler M, Ochocka JR, Sznitowska M. Comparison of the in vitro cytotoxicity among phospholipid-based parenteral drug delivery systems: emulsions, liposomes and aqueous lecithin dispersions (WLDs). Eur. J. Pharm. Sci. 2019; 127: 92-101.