Subject Area and Research Team: V. In vivo studies on mechanism of disea...

Subject Area and Research Team: V. In vivo studies on mechanism of disease and therapy

Research topics

1. Nucleotides metabolism in cardiovascular diseases, neurodegenerative diseases and cancer. Role of microenvironmental factors contributing to tumorigenesis and cancer progression. Application of the mouse models of disease, e.g. lung, breast and pancreatic cancer

2. Modulation of the innate immune system in support of immunotherapy and investigation of the role of G protein-coupled receptor signaling in autoimmune diseases

3. Effect of different treatments on the functions of cardiovascular parameters using experimental disease systems and investigation of cell-penetrating peptides complexes with different drugs to improve PK/PD and decrease toxicity

4. Modification of renal hemodynamics and nephron functions in various pathophysiological conditions

5. The role of fatty acid elongation and lipid alterations in metabolic disease

6. Influence of selected stressors, metabolic and neurodegenerative disorders on the structure and function of the brain tissue


Coordinator #1 Coordinator #2 Coordinator #3 Coordinator #4
Name and surname
Paulina Mierzejewska
Marta Tomczyk
Patrycja Koszałka
Grażyna Peszyńska-Sularz
Academic degree Ph.D. Ph.D. Dr. Habil. V.M.D.
Employment unit Department of Biochemistry Department of Biochemistry Department of Cell Biology and Immunology Tri-City University Animal House – Research Service Center
Polish Platform of Medical Research Paulina Mierzejewska, Ph.D. Marta Tomczyk, Ph.D. Dr. Habil. Patrycja Koszałka Grażyna Anna Peszyńska-Sularz, V.M.D.
Phone number +48 58 523 14 60 +48 58 349 14 60 +48 58 349 14 38 +48 58 349 16 60

Research Team: V. In vivo studies on mechanism of disease and therapy

Team Members: V. In vivo studies on mechanism of disease and therapy (220 KB)

Feel free to contact one of our coordinators to join our Research Team.

Key current projects

1. Energy status and adenine nucleotides metabolism in regulation of cancer progression and promotion, atherosclerosis, heart failure, Huntington’s disease

2. Application of gain-of-function variants of complement C2 protein and investigation of a universal antibody recognizing tumor cells resistant to the first-line immunotherapeutic

3. Examination of conjugates of cell-penetrating peptides with therapeutics in the treatment of diseases, e.g., cancers, Alzheimer’s, Parkinson’s, etc., and investigation of new therapeutic options for pulmonary arterial hypertension

4. Markers of glomerular and tubular dysfunctions in the early and late stages of chronic kidney disease

5. Investigation of lipids alterations in an experimental model of obesity and the role of fatty acid elongation in mice model of ELOVL1 mutation

6. Metabolic and neurodegenerative disorders as determinants of dysfunction of the brain neurovascular unit

Key grants

Funding agency/grant number Title of the project Years
1. NCN, OPUS 19,
Metabolism of very-long-chain fatty acids and pathophysiological role of fatty acid elongase -1 (ELOVL1). 2021-2023
2. NCN, OPUS 18,
Concept of the universal antibody targeting tumor cells resistant to first-line immunotherapeutics 2020-2024
3. NCN, OPUS 17,
The EBI2 receptor/oxysterol signaling in multiple sclerosis pathophysiology, therapy, and diagnostics 2020-2024
The role of AMP deaminase and effects of its inhibition on cardiac mitochondrial function in the experimental hypoxic and failing heart 2019-2021
5. NCN, OPUS 14,
CD73 (ecto-5’-nucleotidase) function in breast cancer progression and promotion, and its assessment as a therapeutic target 2018-2022
6. NCN, OPUS 12,
Adaptations of cardiac myocyte energy metabolism in atherosclerosis – implication for pathology and therapeutic strategies 2017-2021
7. NCN, OPUS 12,
Involvement of P2 receptors in the pathogenesis of glomerular permeability disturbances in experimental-induced diabetes type 1 2017-2021
Involvement of oxidized low-density lipoprotein in the pathogenesis of. glomerular permeability disturbances in hypercholesterolemia 2017-2021
Reversal of heart failure during mechanical support of left ventricular function – the role of nucleotides and energetics in cardiac cells 2017-2022
Functional characteristics and therapeutic potential of gain-of-function mutations in complement C2 protein 2016-2021

International cooperation

Foreign partner (unit name) Principal investigator(s) Area of cooperation
1. Departamento de Bioquímica, Universidade de São Paulo, São Paulo, Brazil Talita Glaser The role of nucleotides metabolism in myopathies related to Huntington’s disease
2. Department of Veterinary Medicine, University of Naples Federico II, Naples, Italy Orlando Paciello Joint funding acquisition, sharing of research tools and expertise
3. Novartis Institutes for Biomedical Research, Cambridge (Massachusetts), USA Andreas Sailer Expertise in GPCR research, support with research tools (e.g. receptor compounds, antibodies, cell lines, knock-out mice, tissue, etc.)
4. German Center for Neurodegenerative Disease, Bonn, Germany Sinead O’Sullivan Joint funding acquisition
5. Department of Life Sciences, Imperial College, London, UK Michał Mielcarek The role of disturbances in cardiac energy and nucleotides in the pathology of Huntington’s disease
6. Radboud University Medical Center, Nijmegen, Netherlands Lambertus van den Heuvel Clincal samples
7. University of Zurich, Zurich, Switzerland Cheryl DeValliere Joint funding acquisition, sharing of research tools and expertise
8. Department of Translational Medicine, Lund University, Lund, Sweden Anna Blom Biacore analysis
9. Department of Molecular Medicine and Surgery Karolinska Institutet, Södertälje, Sweden Camilla Krizhanovskii Sharing research tools and scientific expertise, student supervision
10. NeuroCardioMetabol group, Department of Clinical Science and Education, Karolinska Institutet, Stockholm, Sweden Cesare Patrone Common grants/grant application, sharing research tools and scientific expertise

Key publications

1. Olkowicz M, Tomczyk M, Debski J, Tyrankiewicz U, Przyborowski K, Borkowski T, et al. Enhanced cardiac hypoxic injury in atherogenic dyslipidaemia results from alterations in the energy metabolism pattern. Metabolism. 2021; 114: 154400.

2. Pakiet A, Jakubiak A, Miejerzewska P, Zwara A, Liakh I, Sledzinski T, Mika A. The effect of a high-fat diet on the fatty acid composition in the hearts of mice. Nutients. 2020; 12(3): 824.

3. Urban A, Volokhina E, Felberg A, Stasiłojć G, Blom AM, Jongerius I, van den Heuvel L, Thiel M, Ołdziej S, Arjona E, de Córdoba SR, Okrój M. Gain-of-function mutation in complement C2 protein identified in a patient with aHUS. J Allergy Clin Immunol. 2020; 146: 916-919.e.11.

4. Steliga A, Kowianski P, Czuba E, Waśkow M, Moryś J, Lietzau G. Neurovascular Unit as a Source of Ischemic Stroke Biomarkers-Limitations of Experimental Studies and Perspectives for Clinical Application. Transl Stroke Res. 2020; 11(4): 553-579.

5. Rusiecka I, Ruczyński J, Kozłowska A, Backtrog E, Mucha P, Kocić I, Rekowski P. TP10-Dopamine Conjugate as a Potential Therapeutic Agent in the Treatment of Parkinson’s Disease. Bioconjug Chem. 2019; 30(3): 760-774.

6. Sass P, Sosnowski P, Podolak-Popinigis J, Górnikiewicz B, Kamińska J, Deptuła M, Nowicka E, Wardowska A, Ruczyński J, Rekowski P, Rogujski P, Filipowicz N, Mieczkowska A, Peszyńska-Sularz G, Janus Ł, Skowron P, Czupryn A, Mucha P, Piotrowski A, Rodziewicz-Motowidło S, Pikuła M, Sachadyn P. Epigenetic inhibitor zebularine activates ear pinna wound closure in the mouse. EBioMedicine. 2019; 46: 317-329.

7. Kutryb-Zajac B, Koszalka P, Mierzejewska P, Bulinska A, Zabielska MA, Brodzik K, Skrzypkowska A, Zelazek L, Pelikant-Malecka I, Slominska EM, Smolenski RT. Adenosine deaminase inhibition suppresses progression of 4T1 murine breast cancer by adenosine receptor-dependent mechanisms. J Cell Mol Med. 2018; 22(12): 5939-5954.

8. Kasztan M, Piwkowska A, Kreft E, Rogacka D, Audzeyenka I, Szczepanska-Konkel M, Jankowski M. Extracellular purines’ action on glomerular albumin permeability in isolated rat glomeruli: insights into the pathogenesis of albuminuria. Am J Physiol Renal Physiol. 2016; 311(1): F103-11.